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bceA [2018-02-07 12:27:42]
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bceA [2018-02-07 12:27:42]

primary bacitracin resistance determinant, ABC transporter (ATP-binding protein) for the export of bacitracin, plectasin, mersacidin and actagardine
locus
BSU30380
pI
9.29
mw
28.08 kDa
protein length
253 aa Sequence Blast
gene length
759 bp Sequence Blast
function
export of toxic peptides
product
bacitracin ABC transporter (ATP-binding protein)
essential
no
synonyms
ytsC
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.2|Exporters] → [category|SW 1.2.1.2.3|Export of peptides]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.13|Resistance against toxins/ antibiotics]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    3,111,327 → 3,112,088

    Phenotypes of a mutant

  • 85-fold increased sensitivity to bacitracin [Pubmed|26815905]

    • The protein

    Protein family

  • [SW|ABC transporter] family (according to Swiss-Prot)

    • Effectors of protein activity

  • the activity of the [protein|8E0DE8FE7E9C12495C4DCADA74D838FED088867D|BceA]-[protein|E3613A6C8B5F0EC76510ACE3E478967E73E2B241|BceB] [SW|ABC transporter] is inhibited by heptaprenyl pyrophosphate which accumulates in a ''[gene|596C09DD1D9C305B7C49B2F5E90E2C6C33F63D0E|ytpB]'' mutant [Pubmed|24806199]

    • Structure

  • [PDB|1L2T] (MJ0796 from '' Methanocaldococcus jannaschii '', 38% identity) [Pubmed|12150914]

    • [SW|Localization]

  • membrane associated (via [protein|E3613A6C8B5F0EC76510ACE3E478967E73E2B241|BceB]) [Pubmed|12890034]

    • Expression and Regulation

    Operons

    genes
    [gene|8E0DE8FE7E9C12495C4DCADA74D838FED088867D|bceA]-[gene|E3613A6C8B5F0EC76510ACE3E478967E73E2B241|bceB]
    description
    [Pubmed|12890034]

    regulatory mechanism

  • [protein|C239C155F5452C86BF6C78190BABBBB07A63DF87|BceR]: activation, [Pubmed|12890034], in [regulon|C239C155F5452C86BF6C78190BABBBB07A63DF87|BceR regulon]

    • regulation

  • induced in the presence of bacitracin, plectasin, mersacidin and actagardine, already at very low levels of bacitracin ([protein|search|BceR]) [Pubmed|12890034,26815905]
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    Biological materials

    Mutant

  • MGNA-A167 (ytsC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/167 NBRP B. subtilis, Japan]
  • BKE30380 (Δ[gene|8E0DE8FE7E9C12495C4DCADA74D838FED088867D|bceA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30380 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTACGCAGTCTCCTTTA, downstream forward: _UP4_CAAGGCGTGTTAGGCGGGGT
  • BKK30380 (Δ[gene|8E0DE8FE7E9C12495C4DCADA74D838FED088867D|bceA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30380 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTACGCAGTCTCCTTTA, downstream forward: _UP4_CAAGGCGTGTTAGGCGGGGT

    • Labs working on this gene/protein

  • Foglino M. [Pubmed|17905982]

    • References

    Reviews

  • 27344142,28152228

    • Original publications

  • 17905982,18394148,12890034,14651641,10092453,14612242,21283517,20606066,21078927,23687272,24806199,25118291,26199330,26364265,12150914,27997719,26815905